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Immunohistochemistry of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer, using Phospho-Tau-S404 antibody (AP0170).
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Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-Tau-S404 antibody (AP0170).
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Western blot analysis of extracts of SH-SY5Y and C6 cells, using Phospho-Tau-S404 antibody (AP0170) at 1:2000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100nM) for 1 hour and Calyculin A (100nM) for 30 minutes after serum-starvation overnight.C6 cell lysates were treated by CIP at 37°C for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Phospho-Tau-S404 Rabbit pAb
Catalog No：AB-AP0170
Manufacturer：ABclonal
Synonyms：DDPAC;FTDP-17;MAPTL;MSTD;MTBT1;MTBT2;PPND;PPP1R103;TAU;MAPT;Tau
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Tau-S404 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer’s disease, Pick’s disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S404 of human Tau |
| Gene ID: | 4137 |
| Swiss prot: | P10636 |
| Molecular weight: | 32-45kDa/78- 80kDa |
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