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Western blot analysis of extracts of A-431 cells, using Phospho-PLC gamma 1 (PLCG1)-Y783 antibody (AP0164) at 1:1000 dilution.A-431 cells were treated by EGF (25 ug/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit pAb
Catalog No：AB-AP0164
Manufacturer：ABclonal
Synonyms：PLCG1;NCKAP3;PLC-II;PLC1;PLC148;PLCgamma1
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y783 of human PLC gamma 1 (PLCG1) |
| Gene ID: | 5335 |
| Swiss prot: | P19174 |
| Molecular weight: | 148kDa |
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