Western blot analysis of extracts of HeLa cells, using Phospho-Paxillin-Y118 antibody (AP0156) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-Paxillin-Y118 Rabbit pAb
Catalog No:AB-AP0156
Manufacturer:ABclonal
Synonyms:Paxillin;PXN;paxillin
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-Paxillin-Y118 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y118 of human Paxillin |
Gene ID: | 5829 |
Swiss prot: | P49023 |
Molecular weight: | 46kDa/60kDa/64kDa/66kDa |
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