Western blot analysis of extracts of HeLa cells, using Phospho-STAT4-Y693 antibody (AP0137) at 1:500 dilution.HeLa cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-STAT4-Y693 Rabbit pAb
Catalog No:AB-AP0137
Manufacturer:ABclonal
Synonyms:STAT4;SLEB11
Applications:WB,IHC
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-STAT4-Y693 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y693 of human STAT4 |
Gene ID: | 6775 |
Swiss prot: | Q14765 |
Molecular weight: | 85kDa |
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