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Immunofluorescence analysis of U2OS cells using Phospho-NF-kB p65/RelA-S276 antibody (AP0123) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Phospho-NF-kB p65/RelA-S276 antibody (AP0123) at 1:1000 dilution.HeLa cells,NIH/3T3 cells and C6 cells were treated by TNF-ɑ (20 ng/ml) at 37℃ for 30 minutes.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-NF-kB p65/RelA-S276 Rabbit pAb
Catalog No：AB-AP0123
Manufacturer：ABclonal
Synonyms：NFKB3;p65;NF-kB p65;RELA;CMCU
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-NF-kB p65/RelA-S276 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S276 of human RelA (NP_068810.3). |
| Gene ID: | 5970 |
| Swiss prot: | Q04206 |
| Molecular weight: | 58kDa/59kDa/60kDa |
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