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Western blot analysis of extracts of various cell lines, using Phospho-MAP2K4-T261 pAb (AP0069) at 1:1000 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes or treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-MAP2K4-T261 Rabbit pAb
Catalog No：AB-AP0069
Manufacturer：ABclonal
Synonyms：MAP2K4;JNKK;JNKK1;MAPKK4;MEK4;MKK4;PRKMK4;SAPKK-1;SAPKK1;SEK1;SERK1;SKK1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-MAP2K4-T261 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic phosphorylated peptide around T261 of human MAP2K4 (NP_003001.1). |
| Gene ID: | 6416 |
| Swiss prot: | P45985 |
| Molecular weight: | 44kDa/45kDa |
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