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Western blot analysis of extracts of HeLa cells, using Phospho-MEK1-S217 pAb (AP0062) at 1:1000 dilution or MEK1 antibody (A12687).HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-MEK1-S217 Rabbit pAb
Catalog No：AB-AP0062
Manufacturer：ABclonal
Synonyms：CFC3;MAPKK1;MEK1;MKK1;PRKMK1;MAP2K1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-MEK1-S217 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
| Immunogen: | A synthetic phosphorylated peptide around S217 of human MEK1 (NP_002746.1). |
| Gene ID: | 5604 |
| Swiss prot: | Q02750 |
| Molecular weight: | 40kDa/43kDa |
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