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Western blot analysis of extracts of various cell lines, using Phospho-STAT1-Y701 Rabbit mAb (AP0054) at 1:1000 dilution.HeLa,NIH/3T3 and PC-12 cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-STAT1-Y701 Rabbit mAb
Catalog No：AB-AP0054
Manufacturer：ABclonal
Synonyms：CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;STAT91;STAT1 alpha;STAT1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-STAT1-Y701 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human STAT1. |
| Gene ID: | 6772 |
| Swiss prot: | P42224 |
| Molecular weight: | 84,91kDa |
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