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Western blot analysis of extracts of HeLa cells, using Phospho-eIF4EBP1-S65 antibody (AP0032) at 1:1000 dilution. HeLa cells were treated by IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-eIF4EBP1-S65 Rabbit pAb
Catalog No：AB-AP0032
Manufacturer：ABclonal
Synonyms：4E-BP1;4EBP1;BP-1;PHAS-I;EIF4EBP1
Applications：WB,IP
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-eIF4EBP1-S65 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
| Immunogen: | A synthetic phosphorylated peptide around S65 of human Phospho-eIF4EBP1-S65 (NP_004086.1). |
| Gene ID: | 1978 |
| Swiss prot: | Q13541 |
| Molecular weight: | 12kDa |
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