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Western blot analysis of extracts of 293T cells, using Phospho-eIF4EBP1-T37/46 antibody (AP0030) at 1:1000 dilution.293T were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-eIF4EBP1-T37/46 Rabbit pAb
Catalog No：AB-AP0030
Manufacturer：ABclonal
Synonyms：4E-BP1;4EBP1;BP-1;PHAS-I;EIF4EBP1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-eIF4EBP1-T37/46 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T37/46 of human Phospho-eIF4EBP1-T37/46 |
| Gene ID: | 1978 |
| Swiss prot: | Q13541 |
| Molecular weight: | 12kDa |
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