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Western blot analysis of extracts of various cell lines, using Phospho-ATF2-T71 antibody (AP0020) at 1:1000 dilution. HeLa cells were treated by Anisomycin (25ug/mL) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25ug/mL) for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunofluorescence analysis of MCF-7 cells using Phospho-ATF2-T71 antibody (AP0020). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded rat pancreas using Phospho-ATF2-T71 antibody (AP0020) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-ATF2-T71 antibody (AP0020) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-ATF2-T71 antibody (AP0020) at dilution of 1:100 (40x lens).
Phospho-ATF2-T71 Rabbit pAb
Catalog No：AB-AP0020
Manufacturer：ABclonal
Synonyms：CRE-BP1;CREB-2;CREB2;HB16;TREB7;ATF2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-ATF2-T71 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell’s DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene |
| Immunogen: | A synthetic phosphorylated peptide around T71 of human ATF2 (NP_001871.2). |
| Gene ID: | 1386 |
| Swiss prot: | P15336 |
| Molecular weight: | 13- 15kDa/23-24kDa/35-54kDa |
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