Western blot analysis of over-expressed GST protein using GST-Tag antibody (AE077) at different dilution.Each lane was loaded with 2 ug cell lysate.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Rabbit anti GST-Tag mAb
Catalog No:AB-AE077
Manufacturer:ABclonal
Synonyms:GST;GST tag;GST-tag
Applications:WB,IP
Host:Rabbit
Conjugate:No
€70,00 – €185,00
Product Name: | Rabbit anti GST-Tag mAb |
Category: | Tag and Loading Control Antibodies |
Description: | Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal鈥攁lso known as MAPEG鈥攑roteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. |
Immunogen: | Recombinant protein of GST protein. |
Gene ID: | |
Swiss prot: | |
Molecular weight: | 26kDa |
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