Western blot analysis of extracts of normal 293T cells and 293T transfected with Flag-GSK3B protein,using DDDDK-Tag antibody (AE063) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
DDDDK-Tag Rabbit mAb
Catalog No:AB-AE063
Manufacturer:ABclonal
Synonyms:DDDDK;DDDDK tag;DDDDK-tag
Applications:WB,IHC,IF,IP
Host:Rabbit
Conjugate:No
€110,00 – €325,00
Product Name: | DDDDK-Tag Rabbit mAb |
Category: | Tag and Loading Control Antibodies |
Description: | FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting. |
Immunogen: | A synthetic peptide corresponding to DDDDK tag. |
Gene ID: | |
Swiss prot: | |
Molecular weight: |
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