Western blot analysis of over-expressed DDDDK-tagged protein in 293T cells using Mouse anti DDDDK-Tag antibody (AE005) at different dilution.Each lane was loaded with 2 ug cell lysate.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Mouse anti DDDDK-Tag mAb
Catalog No:AB-AE005
Manufacturer:ABclonal
Synonyms:DDDDK;DDDDK tag;DDDDK-tag
Applications:WB,IHC,IF,IP
Host:Mouse
Conjugate:No
€85,00 – €255,00
Product Name: | Mouse anti DDDDK-Tag mAb |
Category: | Tag and Loading Control Antibodies |
Description: | FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting. |
Immunogen: | A synthetic peptide corresponding to DDDDK tag. |
Gene ID: | |
Swiss prot: | |
Molecular weight: |
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