Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein,using His-Tag antibody (AE003) at 1:100000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 120s.
Western blot analysis of extracts of normal 293T cells and 293T transfected with EPCAM protein,using His-Tag antibody (AE003) at 1:20000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Mouse anti His-Tag mAb
Catalog No:AB-AE003
Manufacturer:ABclonal
Synonyms:His;His tag;His-tag
Applications:WB,IF,IP
Host:Mouse
Conjugate:No
€70,00 – €765,00
Product Name: | Mouse anti His-Tag mAb |
Category: | Tag and Loading Control Antibodies |
Description: | Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies. |
Immunogen: | A synthetic peptide corresponding to His tag. |
Gene ID: | |
Swiss prot: | |
Molecular weight: |
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