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Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (A10613) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
ERK1 / ERK2 Mouse mAb
Catalog No：AB-A10613
Manufacturer：ABclonal
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Mouse
Conjugate：No
€195,00 – €540,00
| Product Name: | ERK1 / ERK2 Mouse mAb |
| Category: | Monoclonal Antibodies |
| Description: | MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. |
| Immunogen: | A synthetic peptide of human ERK1 / ERK2 |
| Gene ID: | 5594/5595 |
| Swiss prot: | P28482/P27361 |
| Molecular weight: | 36kDa/41kDa/38kDa/40kDa/43kDa |
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