Western blot analysis of extracts of various cell lines, using CAPN10 antibody (A9865) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
CAPN10 Rabbit pAb
Catalog No:AB-A9865
Manufacturer:ABclonal
Synonyms:CAPN10;CANP10;NIDDM1
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CAPN10 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CAPN10 (NP_075571.1). |
Gene ID: | 11132 |
Swiss prot: | Q9HC96 |
Molecular weight: | 15kDa/29kDa/49kDa/57kDa/60kDa/74kDa |
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