Western blot analysis of extracts of various cell lines, using ZCCHC8 antibody (A9563) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
ZCCHC8 Rabbit pAb
Catalog No:AB-A9563
Manufacturer:ABclonal
Synonyms:ZCCHC8
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ZCCHC8 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a scaffold protein which serves as an assessory factor to the nuclear RNA exosome complex. The encoded protein forms a trimeric human nuclear exosome targeting (NEXT) complex, together with hMTR4 and the RNA-binding factor RBM7 which promotes the exosomal degradation of non-coding promoter-upstream transcripts, enhancer RNAs and 3′-extended products of histone- and small nuclear RNA transcription. This complex is also thought to recruit the exosome to degrade intronic RNAs via its interaction with both the exosome and the spliceosome. It contains both an N-terminal zinc-knuckle domain and a C-terminal proline-rich domain. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZCCHC8 (NP_060082.2). |
Gene ID: | 55596 |
Swiss prot: | Q6NZY4 |
Molecular weight: | 51kDa/78kDa |
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