Western blot analysis of extracts of various cell lines, using SNF8 antibody (A10583) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
SNF8 Rabbit pAb
Catalog No:AB-A10583
Manufacturer:ABclonal
Synonyms:SNF8;Dot3;EAP30;VPS22
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SNF8 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNF8 (NP_009172.2). |
Gene ID: | 11267 |
Swiss prot: | Q96H20 |
Molecular weight: | 28kDa |
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