Western blot analysis of extracts of various cell lines, using Cortactin Rabbit mAb (A9518) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Cortactin Rabbit mAb
Catalog No:AB-A9518
Manufacturer:ABclonal
Synonyms:EMS1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | Cortactin Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010] |
Immunogen: | A synthesized peptide derived from human Cortactin |
Gene ID: | 2017 |
Swiss prot: | Q14247 |
Molecular weight: | 80kDa |
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