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Western blot analysis of extracts of various cell lines, using 14-3-3 epsilon antibody (A1058) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
14-3-3 epsilon Rabbit pAb
Catalog No：AB-A1058
Manufacturer：ABclonal
Synonyms：YWHAE;14-3-3E;HEL2;KCIP-1;MDCR;MDS
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | 14-3-3 epsilon Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human 14-3-3 epsilon (NP_006752.1). |
| Gene ID: | 7531 |
| Swiss prot: | P62258 |
| Molecular weight: | 26kDa/29kDa |
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