Western blot analysis of extracts of various cell lines, using MRPS12 antibody (A10573) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
MRPS12 Rabbit pAb
Catalog No:AB-A10573
Manufacturer:ABclonal
Synonyms:MRPS12;MPR-S12;MT-RPS12;RPMS12;RPS12;RPSM12
Applications:WB,IHC
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MRPS12 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5′ UTR have been found for this gene; all three variants encode the same protein. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-138 of human MRPS12 (NP_203527.1). |
Gene ID: | 6183 |
Swiss prot: | O15235 |
Molecular weight: | 15kDa |
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