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Western blot analysis of extracts of various cell lines, using UBE2M antibody (A9007) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
UBE2M Rabbit pAb
Catalog No：AB-A9007
Manufacturer：ABclonal
Synonyms：UBE2M;UBC-RS2;UBC12;hUbc12
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UBE2M Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2M (NP_003960.1). |
| Gene ID: | 9040 |
| Swiss prot: | P61081 |
| Molecular weight: | 20kDa |
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