Western blot analysis of extracts of various cell lines, using MED17 antibody (A8716) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using MED17 antibody (A8716) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
MED17 Rabbit pAb
Catalog No:AB-A8716
Manufacturer:ABclonal
Synonyms:MED17;CRSP6;CRSP77;DRIP80;TRAP80;SRB4
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MED17 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MED17 (NP_004259.3). |
Gene ID: | 9440 |
Swiss prot: | Q9NVC6 |
Molecular weight: | 15kDa/72kDa |
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