Western blot analysis of extracts of Raji cells, using Human IgM antibody (A8677) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Human IgM Rabbit pAb
Catalog No:AB-A8677
Manufacturer:ABclonal
Synonyms:IGHM;AGM1;MU;VH
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€245,00 – €370,00
Product Name: | Human IgM Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes, joining (J) genes, and diversity (D) genes. The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma. |
Immunogen: | A synthetic Peptide of human IGHM |
Gene ID: | 3507 |
Swiss prot: | P01871 |
Molecular weight: | 49kDa/51kDa |
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