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Western blot analysis of extracts of various cell lines, using LGR5 antibody (A10545) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Western blot analysis of extracts of various cell lines, using LGR5 antibody (A10545) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using LGR5/GPR49 Rabbit pAb (A10545) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human oophoroma using LGR5/GPR49 Rabbit pAb (A10545) at dilution of 1:200 (40x lens).
LGR5/GPR49 Rabbit pAb
Catalog No：AB-A10545
Manufacturer：ABclonal
Synonyms：LGR5;FEX;GPR49;GPR67;GRP49;HG38
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | LGR5/GPR49 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 824-907 of human LGR5/GPR49 (NP_003658.1). |
| Gene ID: | 8549 |
| Swiss prot: | O75473 |
| Molecular weight: | 92kDa/97kDa/99kDa |
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