Western blot analysis of extracts of various cell lines, using BCCIP antibody (A8586) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
BCCIP Rabbit pAb
Catalog No:AB-A8586
Manufacturer:ABclonal
Synonyms:BCCIP;TOK-1;TOK1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | BCCIP Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human BCCIP (NP_510868.1). |
Gene ID: | 56647 |
Swiss prot: | Q9P287 |
Molecular weight: | 33kDa/35kDa/36kDa |
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