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Western blot analysis of extracts of various cell lines, using SART1 antibody (A8569) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human esophageal using SART1 Rabbit pAb (A8569) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using SART1 Rabbit pAb (A8569) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using SART1 Rabbit pAb (A8569) at dilution of 1:100 (40x lens).
SART1 Rabbit pAb
Catalog No：AB-A8569
Manufacturer：ABclonal
Synonyms：SART1;Ara1;HOMS1;SART1259;SNRNP110;Snu66;HAF
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | SART1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1). |
| Gene ID: | 9092 |
| Swiss prot: | O43290 |
| Molecular weight: | 90kDa |
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