Western blot analysis of extracts of various cell lines, using L1CAM antibody (A8555) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts from normal (control) and L1CAM knockout (KO) HeLa cells, using L1CAM antibody (A8555) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] L1CAM Rabbit pAb
Catalog No:AB-A8555
Manufacturer:ABclonal
Synonyms:L1CAM;CAML1;CD171;HSAS;HSAS1;MASA;MIC5;N-CAM-L1;N-CAML1;NCAM-L1;S10;SPG1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] L1CAM Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1143-1257 of human L1CAM (NP_000416.1). |
Gene ID: | 3897 |
Swiss prot: | P32004 |
Molecular weight: | 138kDa/139kDa/140kDa |
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