Western blot analysis of extracts of various cell lines, using RNF34 antibody (A8517) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
RNF34 Rabbit pAb
Catalog No:AB-A8517
Manufacturer:ABclonal
Synonyms:RNF34;CARP-1;CARP1;RFI;RIF;RIFF;hRFI
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RNF34 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RNF34 (NP_079402.2). |
Gene ID: | 80196 |
Swiss prot: | Q969K3 |
Molecular weight: | 41kDa |
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