Western blot analysis of extracts of MCF-7 cells, using REV1 antibody (A8493) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
REV1 Rabbit pAb
Catalog No:AB-A8493
Manufacturer:ABclonal
Synonyms:REV1;AIBP80;REV1L
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | REV1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-345 of human REV1 (NP_057400.1). |
Gene ID: | 51455 |
Swiss prot: | Q9UBZ9 |
Molecular weight: | 43kDa/138kDa |
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