Western blot analysis of extracts of various cell lines, using HIRA antibody (A8461) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts from normal (control) and HIRA Rabbit pAb knockout (KO) 293T cells, using HIRA Rabbit pAb antibody (A8461) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
[KO Validated] HIRA Rabbit pAb
Catalog No:AB-A8461
Manufacturer:ABclonal
Synonyms:HIRA;DGCR1;TUP1;TUPLE1
Applications:WB,IHC
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] HIRA Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3). |
Gene ID: | 7290 |
Swiss prot: | P54198 |
Molecular weight: | 89kDa/111kDa |
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