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Western blot analysis of extracts of various cell lines, using SIGLEC8 antibody (A10514) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
SIGLEC8 Rabbit pAb
Catalog No：AB-A10514
Manufacturer：ABclonal
Synonyms：SIGLEC8;SAF2;SIGLEC-8;SIGLEC8L
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | SIGLEC8 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010] |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human SIGLEC8 (NP_055257.2). |
| Gene ID: | 27181 |
| Swiss prot: | Q9NYZ4 |
| Molecular weight: | 44kDa/46kDa/54kDa |
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