Western blot analysis of extracts of various cell lines, using CLDN18 antibody (A8392) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
CLDN18 Rabbit pAb
Catalog No:AB-A8392
Manufacturer:ABclonal
Synonyms:CLDN18;SFTA5;SFTPJ
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CLDN18 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-80 of human CLDN18 (NP_057453.1). |
Gene ID: | 51208 |
Swiss prot: | P56856 |
Molecular weight: | 27kDa |
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