Western blot analysis of extracts of HT-1080 cells, using METTL3 antibody (A8370) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using METTL3 antibody (A8370) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of Rat spleen, using METTL3 antibody (A8370) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Western blot analysis of extracts from normal (control) and METTL3 knockout (KO) 293T cells, using METTL3 antibody (A8370) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
METTL3 Rabbit pAb
Catalog No:AB-A8370
Manufacturer:ABclonal
Synonyms:IME4;M6A;MT-A70;Spo8;hMETTL3;METTL3
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | METTL3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2). |
Gene ID: | 56339 |
Swiss prot: | Q86U44 |
Molecular weight: | 25kDa/64kDa |
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