Western blot analysis of extracts of various cell lines, using POFUT1 antibody (A8338) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
POFUT1 Rabbit pAb
Catalog No:AB-A8338
Manufacturer:ABclonal
Synonyms:POFUT1;DDD2;FUT12;O-FUT;O-Fuc-T;O-FucT-1;OFUCT1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | POFUT1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-194 of human POFUT1 (NP_758436.1). |
Gene ID: | 23509 |
Swiss prot: | Q9H488 |
Molecular weight: | 22kDa/43kDa |
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