Western blot analysis of extracts of various cell lines, using MID2 antibody (A8325) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 40s.
MID2 Rabbit pAb
Catalog No:AB-A8325
Manufacturer:ABclonal
Synonyms:MID2;FXY2;MRX101;RNF60;TRIM1;midline 2
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MID2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 476-705 of human MID2 (NP_438112.2). |
Gene ID: | 11043 |
Swiss prot: | Q9UJV3 |
Molecular weight: | 79kDa/83kDa |
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