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Western blot analysis of extracts of various cell lines, using ALOXE3 antibody (A8245) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
ALOXE3 Rabbit pAb
Catalog No：AB-A8245
Manufacturer：ABclonal
Synonyms：ALOXE3;ARCI3;E-LOX;eLOX-3;eLOX3
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ALOXE3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. This gene is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ALOXE3 (NP_067641.2). |
| Gene ID: | 59344 |
| Swiss prot: | Q9BYJ1 |
| Molecular weight: | 80kDa/95kDa |
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