Western blot analysis of extracts of various cell lines, using NDUFA12 antibody (A8237) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
NDUFA12 Rabbit pAb
Catalog No:AB-A8237
Manufacturer:ABclonal
Synonyms:NDUFA12;B17.2;DAP13
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NDUFA12 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human NDUFA12 (NP_061326.1). |
Gene ID: | 55967 |
Swiss prot: | Q9UI09 |
Molecular weight: | 7kDa/17kDa |
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