Western blot analysis of extracts of various cell lines, using RNF31 antibody (A8227) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
RNF31 Rabbit pAb
Catalog No:AB-A8227
Manufacturer:ABclonal
Synonyms:RNF31;HOIP;Paul;ZIBRA
Applications:WB,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RNF31 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human RNF31 (NP_060469.4). |
Gene ID: | 55072 |
Swiss prot: | Q96EP0 |
Molecular weight: | 57kDa/102kDa/119kDa |
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