Western blot analysis of extracts of various cell lines, using ARFGAP3 antibody (A8204) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
ARFGAP3 Rabbit pAb
Catalog No:AB-A8204
Manufacturer:ABclonal
Synonyms:ARFGAP3;ARFGAP1
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ARFGAP3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 217-516 of human ARFGAP3 (NP_055385.3). |
Gene ID: | 26286 |
Swiss prot: | Q9NP61 |
Molecular weight: | 51kDa/56kDa |
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