Western blot analysis of extracts of 22Rv1 cells, using BCKDK antibody (A8184) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
BCKDK Rabbit pAb
Catalog No:AB-A8184
Manufacturer:ABclonal
Synonyms:BCKDK;BCKDKD;BDK
Applications:WB,IHC,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | BCKDK Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 153-412 of human BCKDK (NP_005872.2). |
Gene ID: | 10295 |
Swiss prot: | O14874 |
Molecular weight: | 37kDa/41kDa/46kDa |
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