Western blot analysis of extracts of various cell lines, using DDIT4 antibody (A8086) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Western blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody (A8086) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
[KO Validated] DDIT4 Rabbit pAb
Catalog No:AB-A8086
Manufacturer:ABclonal
Synonyms:DDIT4;Dig2;REDD-1;REDD1
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] DDIT4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1). |
Gene ID: | 54541 |
Swiss prot: | Q9NX09 |
Molecular weight: | 25kDa |
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