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Western blot analysis of extracts of various cell lines, using ANAPC2 antibody (A8066) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
ANAPC2 Rabbit pAb
Catalog No：AB-A8066
Manufacturer：ABclonal
Synonyms：ANAPC2;APC2
Applications：WB
Reactivity：Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ANAPC2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human ANAPC2 (NP_037498.1). |
| Gene ID: | 29882 |
| Swiss prot: | Q9UJX6 |
| Molecular weight: | 93kDa |
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