Western blot analysis of extracts of various cell lines, using USP25 antibody (A7975) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
USP25 Rabbit pAb
Catalog No:AB-A7975
Manufacturer:ABclonal
Synonyms:USP25;USP21
Applications:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | USP25 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_037528.3). |
Gene ID: | 29761 |
Swiss prot: | Q9UHP3 |
Molecular weight: | 122kDa/125kDa/129kDa |
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