Western blot analysis of extracts of various cell lines, using OCT4 antibody (A7920) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
OCT4 Rabbit pAb
Catalog No:AB-A7920
Manufacturer:ABclonal
Synonyms:POU5F1;OCT3;OCT4;OTF-3;OTF3;OTF4;Oct-3;Oct-4
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | OCT4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing’s sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. |
Immunogen: | A synthetic peptide of human OCT4 |
Gene ID: | 5460 |
Swiss prot: | Q01860 |
Molecular weight: | 30kDa/38kDa |
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