Western blot analysis of extracts of various cell lines, using UBE2A antibody (A7744) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
UBE2A Rabbit pAb
Catalog No:AB-A7744
Manufacturer:ABclonal
Synonyms:UBE2A;HHR6A;MRXS30;MRXSN;RAD6A;UBC2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | UBE2A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2). |
Gene ID: | 7319 |
Swiss prot: | P49459 |
Molecular weight: | 8kDa/13kDa/17kDa |
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