Western blot analysis of extracts of various cell lines, using MED14 antibody (A10455) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
MED14 Rabbit pAb
Catalog No:AB-A10455
Manufacturer:ABclonal
Synonyms:MED14;CRSP150;CRSP2;CSRP;CXorf4;DRIP150;EXLM1;RGR1;TRAP170
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MED14 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-650 of human MED14 (NP_004220.2). |
Gene ID: | 9282 |
Swiss prot: | O60244 |
Molecular weight: | 160kDa |
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