Western blot analysis of extracts of various cell lines, using HLA-DRB1 antibody (A7685) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.
HLA-DRB1 Rabbit pAb
Catalog No:AB-A7685
Manufacturer:ABclonal
Synonyms:HLA-DRB1;DRB1;HLA-DR1B;HLA-DRB;SS1;major histocompatibility complex;class II;DR beta 1
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HLA-DRB1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human HLA-DRB1 (NP_002115.2). |
Gene ID: | 3123 |
Swiss prot: | P01911/P01912/Q29974 |
Molecular weight: | 30kDa |
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