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Western blot analysis of extracts of various cell lines, using Chk1 antibody (A7653) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of HT-29 cells, using Chk1 antibody (A7653) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded Mouse spleen using Chk1 Rabbit pAb (A7653) at dilution of 1:50 (40x lens).
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Immunohistochemistry of paraffin-embedded Rat spleen using Chk1 Rabbit pAb (A7653) at dilution of 1:50 (40x lens).
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Immunohistochemistry of paraffin-embedded Human esophageal cancer using Chk1 Rabbit pAb (A7653) at dilution of 1:50 (40x lens).
Chk1 Rabbit pAb
Catalog No：AB-A7653
Manufacturer：ABclonal
Synonyms：CHEK1;CHK1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Chk1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. |
| Immunogen: | A synthetic peptide of human Chk1 |
| Gene ID: | 1111 |
| Swiss prot: | O14757 |
| Molecular weight: | 43kDa/50kDa/54kDa |
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